uvrC [2020-09-15 12:43:20]

excinuclease ABC (subunit C), required for transcription-dependent asymmetry in mutation rates of genes in the two orientations

Locus: BSU_28490

Isoelectric point: 9.50

Molecular weight: 69.28 kDa

Protein length: 590 aa

Gene length: 1773 bp

Function: DNA repair after UV damage

Product: excinuclease ABC (subunit C)

Essential: no

Synonyms: uvrB

Genomic Context

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Categories containing this gene/protein

• Information processing → Genetics → DNA repair/ recombination
• Information processing → Genetics → DNA repair/ recombination → Other proteins

Gene

Coordinates: 2,911,116 2,912,888

The protein

Protein family

• uvrC family (single member, according to UniProt)

Domains

• GIY-YIG-domain (aa 14-91) (according to UniProt)
• UVR domain (aa 196-231) (according to UniProt)

Structure

• 3C65 (5' endonuclease domain, Geobacillus stearothermophilus)

Expression and Regulation

Operons

• Genes: uvrC
  Description: PubMed

  Regulatory mechanism

  • LexA: repression, PubMed], in LexA regulon

  Regulation

  • induced upon DNA damage (LexA) PubMed

Biological materials

Mutant

• BKE28490 (uvrC::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATATGTTACCCTTCCTTAT, downstream forward: _UP4_TAATGTTGTCCTTTTAAATA
• BKK28490 (uvrC::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATATGTTACCCTTCCTTAT, downstream forward: _UP4_TAATGTTGTCCTTTTAAATA

References

Reviews

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Original publications

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